What is
DNA?
DNA is material that governs inheritance of eye color,
hair color, stature, bone density and many other human
and animal traits. DNA is a long, but narrow string-like
object. A one foot long string or strand of DNA is normally
packed into a space roughly equal to a cube 1/millionth
of an inch on a side. This is possible only because
DNA is a very thin string.
Our body's cells each contain a complete sample of
our DNA. One cell is roughly equal in size to the cube
described in the previous paragraph. There are muscle
cells, brain cells, liver cells, blood cells, sperm
cells and others. Basically, every part of the body
is made up of these tiny cells and each contains a sample
or complement of DNA identical to that of every other
cell within a given person. There are a few exceptions.
For example, our red blood cells lack DNA. Blood itself
can be typed because of the DNA contained in our white
blood cells.
Not only does the human body rely on DNA but so do
most living things including plants, animals and bacteria.
A strand of DNA is made up of tiny building-blocks.
There are only four, different basic building-blocks.
Scientists usually refer to these using four letters,
A, T, G, and C. These four letters are short nicknames
for more complicated building-block chemical names,
but actually the letters (A,T, G and C) are used much
more commonly than the chemical names so the latter
will not be mentioned here. Another term for DNA's building
blocks is the term, "bases." A, T, G and C
are bases.
For example, to refer to a particular piece of DNA,
we might write: AATTGCCTTTTAAAAA. This is a perfectly
acceptable way of describing a piece of DNA. Someone
with a machine called a DNA synthesizer could actually
synthesize the same piece of DNA from the information
AATTGCCTTTTAAAAA alone.
The sequence of bases (letters) can code for many properties
of the body's cells. The cells can read this code. Some
DNA sequences encode important information for the cell.
Such DNA is called, not surprisingly, "coding DNA."
Our cells also contain much DNA that doesn't encode
anything that we know about. If the DNA doesn't encode
anything, it is called non-coding DNA or sometimes,
"junk DNA."[1]
The DNA code, or genetic code as it is called, is passed
through the sperm and egg to the offspring. A single
sperm cell contains about three billion bases consisting
of A, T, G and C that follow each other in a well defined
sequence along the strand of DNA. Each egg cell also
contains three billion bases arranged in a well-defined
sequence very similar, but not identical to the sperm.
Both coding and non-coding DNAs may vary from one individual
to another. These DNA variations can be used to identify
people or at least distinguish one person from another.
What is a Locus?
A locus (with a hard "c", LOW-KUS) is simply
a location in the DNA. The plural of locus is, loci
( with a soft "c", pronounced LOW-S-EYE).
Again, the DNA is a long string like object as illustrated
below. A locus is simply a location in the DNA. Such
locations, or loci, reside at specific places on chromosomes.
What is a Chromosome?
When a cell is getting ready to divide creating two
daughter cells, it packs its DNA into bundles called
chromosomes. Chromosomes are just bundles of DNA. For
humans, there are consistently 23 pairs of chromosomes,
each with a consistent size and shape. Chromosomes are
numbered. Chromosome number 1 is the largest chromosome;
chromosome number 2 a little smaller and so on. Among
the 23 pairs of chromosomes there is a pair called the
sex chromosomes. This is something of a misnomer, since
there are many functions on the "sex" chromosomes
that have nothing to do with sex. In females, the sex-chromosome
pair consists of two similar size chromosomes called
X chromosomes. Males have one X and one sma
What is DNA Profiling or DNA test?
DNA profiling (also called DNA testing, DNA typing,
or genetic fingerprinting) is a technique employed by
forensic scientists to assist in the identification
of individuals on the basis of their respective DNA
profiles. DNA testing compares genetic material from
two or more people to determine if they are biologically
related. The genetic material for testing is usually
taken from a mouth swab or blood sample.
How the profiling is done?
DNA profiling commences with the extraction of an individual's
DNA (typically called a "reference sample").
The most desirable method of collecting a reference
sample is the use of a buccal swab, as this reduces
the possibility of contamination. When this is not available
(eg because a court order may be needed and not obtainable)
other methods may need to be used to collect a sample
of blood, saliva, semen, or other appropriate fluid
or tissue from personal items (e.g. toothbrush, razor,
etc) or from stored samples (e.g. banked sperm or biopsy
tissue). Samples obtained from blood relatives (biological
relative) can provide an indication of an individual's
profile, as could human remains which had been previously
profiled.
A reference sample is then analysed to create the individual's
DNA profile using one of a number of techniques.
Why the Genealogical DNA test is done?
A genealogical DNA test examines the nucleotides at
specific locations on a person's DNA for genetic genealogy
purposes. The test results are meant to have no informative
medical value and do not determine specific genetic
diseases or disorders (see possible exceptions in Medical
information below); they are intended only to give genealogical
information. Genealogical DNA tests generally involve
comparing the results of living individuals as opposed
to obtaining samples from deceased people.
Courtesy from the follwing websites. Please visit further
for more information on DNA Tests.
http://www.scientific.org/tutorials/articles/riley/riley.html
http://en.wikipedia.org/wiki/Genetic_fingerprinting
http://en.wikipedia.org/wiki/Genealogical_DNA_test
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